Aim and Background: Colorectal cancer is the third most common cancer after lung cancer, stomach and liver cancer in the world. Studies have shown that patients with inflammatory disease of the large intestine are at high risk of developing colorectal cancer are located. Several factors make inflammatory bowel disease and colorectal cancer is one of the factors involved, bacteria and toxins derived from them. Research shows that some strains of E. coli can be induced to increase the mutation rate that was established in 2010 by Cuvas-Romas and colleagues. fimC and vat1 Virulence genes in these organisms play an important role in bacteria pathogenicity. This study is to compare the virulence genes in E.coli strains isolated from samples biopsy patients’ inflammatory bowel disease and colorectal cancer, intestinal tissue was performed.Materialsand Methods: 38 biopsies were obtained from intestinal tissue and E.coli bacteria in microbial and biochemical method was identified and isolated. After DNA extraction, strains for virulence genes vat 1, fim C using polymerase chain reaction (PCR), were evaluated.Results: Molecular analysis showed a significant difference between the groups studied the gene vat 1 (p=0.0245) and 42.8% of positive samples were for this gene in normal groups, 87.5% with inflammatory bowel disease87.5 % of patients with colorectal cancer were reported. But there is no significant difference between the groups in terms of fim C gene (P=0.201). And 71.4% of positive samples for this gene in normal groups’ and 93.7% in patients with inflammatory bowel disease73.3 % of patients with colorectal cancer were reported.Conclusion: The results, to confirm the good relationship between virulence genes studied induced inflammation and proliferation by inducing mutation rate.

Objectives: Bordetella pertussis is a Gram-negative coccobacillus bacterium which is the causative agent of whooping cough. In recent years, the number of whooping cough cases has been rised. This bacterium has important virulence factors such as fimbriae and pertactin. In this study, polymorphism of Serotype 2 and 3 fimbriae genes and 2 Regions of pertactin gene were surveyed. Materials & Methods: Totally, 20 B. pertussis clinical isolates were tested. DNA was extracted using the kit. Serotypes 2 and 3 fimbriae genes and pertactin Region 1 and 2 were identified using PCR method; finally, 13 samples were randomly sequenced. Findings: No mutation was observed in the pertactin Region 2. In relation to the region1 of pertactin, %77 and %23 of the strains had prn2 and prn1 alleles, respectively. In relation to fim2 gene, %70 and %30 of the strains had fim2-2 and fim1-2 alleles, respectively. Also, in relation to fim3 gene, %70 and %30 of the strains carried fim3B and fim3A alleles, respectively. Conclusion: In general, the present study results were similar to those of the previous studies conducted in Iran, but there were some differences in fim2 gene polymorphism so that the dominant allele changed from fim1-2 to fim2-2. Considering the fact that vaccine strains of Bp134 and Bp509 carry fim3A allele, which is different from the dominant circulating allele (fim3B), it is suggested that strains more similar to the dominant circulating strains should be used in designing vaccines.

Background and objectives: Urinary tract infections (UTIs) are one of the most common infectious diseases caused by bacteria. The primary etiologic agent of UTIs is Escherichia coli. Uropathogenic E. coli (UPEC) strains have a number of specific virulence factors, which can worsen UTIs. This study was performed to detect fim, pap, sfa and afa genes among E. coli strains isolated from UTIs. Methods: A total of 100 E. coli isolates from patients with UTI was collected between June and December 2015 from Mosavi and Sayyad Shirazi hospitals in Gorgan, Iran. All bacterial isolates were identified via standard biochemical testing and Gram straining. Presence of the genes was assessed by polymerase chain reaction. Results: The frequency of the fim, pap, sfa and afa genes was 100%, 79%, 69% and 8%, respectively. All isolates contained at least one virulence gene. Prevalence of multiple adhesion genes was 6% for all genes and 65% for three genes (fim, pap and sfa) together. In addition, the frequency of the fim gene was significantly higher than that of the other genes (P<0. 0001). Conclusion: The results of this study indicate the high prevalence of virulence factors that can enhance pathogenicity of E. coli. Therefore, these factors could be used as diagnostic markers or vaccine targets.